Collectively, our findings demonstrate that compound 1b notably induces mitochondrial-mediated apoptosis in K 562 cells, which might have a potential anticancer activity. Furthermore, we observed dissipation of the mitochondrial membrane potential, caspase-3 activation, and a reduction of the Bcl-2/Bax ratio in these cells, which suggest that the mitochondrial apoptotic pathway is induced by 1b in K 562 cells. Flow cytometric analysis also confirmed the presence of an apoptotic cell population following treatment of Annexin-V-FITC and propidium iodide (PI) double-labeled K 562 cells with 1b. Treatment with compound 1b caused K 562 cells to adopt a typical apoptotic morphology. In the present study, we investigated the molecular mechanism underlying the cytotoxicity of 1b in K 562 cells. Teng, Yuou Wang, Lixin Liu, Huan Yuan, Yuan Zhang, Qian Wu, Meng Wang, Luyao Wang, Haomeng Liu, Zhen Yu, Pengģ'-Geranyl-mono-substituted chalcone Xanthoangelol (1b), a chalcone derivative, was previously reported to show selective cytotoxicity against human chronic myelogenous leukemia K 562 cells with a half-maximal inhibitory concentration ( IC 50 ) of 3.98 μM. The aptamer-siRNA compound can significantly induce K 562 cell apoptosis, and provide reference for gene therapy of patients with chronic myelocytic lenkemia.ģ'-Geranyl-mono-substituted chalcone Xanthoangelovl induces apoptosis in human leukemia K 562 cells via activation of mitochondrial pathway.
According to agarose gel electrophoresis observation, when the aptamer-siRNA compound showed effect on K 562 cells, the typical DNA lader could be observed. The MTT assay showed that the IC 50 value of aptamer-siRNA compound for K 562 cells was 150 µmol/L.
As compared with control, the aptamer-siRNA compound showed more inhibitory effect on K 562 cells and there was significant difference (P<0.05). The remarkably changes of morphology and structure of K 562 cells treated with 200 µmol/L aptamer-siRNA were observed under fluorescence microscopy and electromicroscopy. the changes of cellular morphology and structure were observed by using fluorescence microscope, laser confocal microscope, JEM-4000EX transmission electron microscopy MTT assay were performed to evaluate the sensibility of K 562 cells to aptamer-siRNA compound, the apoptosis was detected by DNA gel electro-phoresis. To explore the effects of aptamer-siRNA nucleic acid compound on growth and apoptosis in myeloid leukemia cell line K 562. Ping, Juan Shen, Zhi-Hui Wang, Bao-Quan Zhao, Na Li, Rui Li, Mian Pang, Xiao-Bin Chen, Chuan-Bo
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